LSBio
Polyclonal Rabbit anti-Human TRIM54 / MURF Antibody (WB)

Western blot analysis of extracts of various cell lines, using TRIM54 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.

Polyclonal Rabbit anti-Human TRIM54 / MURF Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human TRIM54 / MURF antibodies →
$281.00each
Catalog Number: LS-C748169-20

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Overview

Description
MURF antibody LS-C748169 is an unconjugated rabbit polyclonal antibody to MURF (TRIM54) from human. It is reactive with human and mouse. Validated for WB.
Application
WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
MURF | MURF-3 | MuRF3 | RING finger protein 30 | TRIM54 | Tripartite motif-containing 54 | RNF30 | Tripartite motif containing 54
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q9BYV2
NCBI GenBank
NM_032546 | NP_115935.3
SwissProt
Q9BYV2

Target

Target
Human TRIM54 / MURF
Antigen Species
Human
Gene Name
TRIM54

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human TRIM54 (NP_912730.2). AMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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