LSBio
Polyclonal Rabbit anti-Human TOP2A / Topoisomerase II Alpha Antibody (IHC, IF, WB)

Western blot analysis of extracts of various cell lines, using TOP2A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

Polyclonal Rabbit anti-Human TOP2A / Topoisomerase II Alpha Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human TOP2A / Topoisomerase II Alpha antibodies →
$281.00each
Catalog Number: LS-C331089-20

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Overview

Description
Topoisomerase II Alpha antibody LS-C331089 is an unconjugated rabbit polyclonal antibody to human Topoisomerase II Alpha (TOP2A). Validated for IF, IHC and WB.
Application
IF, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DNA topoisomerase II, 170 kD | TOP2 | TOP2A | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
Recommended Dilution: IF/ICC
1:20 - 1:50
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P11388
NCBI GenBank
NM_001067 | NP_001058.2
SwissProt
P11388

Target

Target
Human TOP2A / Topoisomerase II Alpha
Antigen Species
Human
Epitope
Human TOP2A / Topoisomerase II Alpha
Gene Name
TOP2A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
A synthetic peptide of human TOP2A.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C, avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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