LSBio
Polyclonal Rabbit anti-Human TBX21 / T-bet Antibody (aa22-50, IF, WB)

Fluorescent image of A549 cell stained with TBX21 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX21 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). TBX21 immunoreactivity is localized to Nucleus significantly.

Polyclonal Rabbit anti-Human TBX21 / T-bet Antibody (aa22-50, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human TBX21 / T-bet antibodies →
$401.00each
Catalog Number: LS-C161751-400

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Overview

Description
T-bet antibody LS-C161751 is an unconjugated rabbit polyclonal antibody to human T-bet (TBX21) (aa22-50). Validated for IF and WB.
Application
IF, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
T-bet | TBET | T-box 21 | T-box protein 21 | TBLYM | Transcription factor TBLYM | T-box expressed in T cells | TBX21 | T-PET
Recommended Dilution: IF/ICC
1:10 - 1:50
Recommended Dilution: WB
1:1000
UniProt Number
Q9UL17
SwissProt
Q9UL17

Target

Target
Human TBX21 / T-bet
Antigen Species
Human
Epitope
This TBX21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-50 amino acids from the N-terminal region of human TBX21.
Gene Name
TBX21

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
5 mg/mL
Volume
400 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS

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