LSBio
Polyclonal Rabbit anti-Human STUB1 / CHIP Antibody (IHC, IF, WB)

Positive WB detected in:A549 whole cell lysate,Rat brain tissue,Mouse brain tissue;All lanes:STUB1 antibody at 2?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 35,28 KDa;Observed band size: 35,28 KDa;

Polyclonal Rabbit anti-Human STUB1 / CHIP Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human STUB1 / CHIP antibodies →
$300.00each
Catalog Number: LS-C502171-50

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Overview

Description
CHIP antibody LS-C502171 is an unconjugated rabbit polyclonal antibody to CHIP (STUB1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Antigen NY-CO-7 | CHIP | HSPABP2 | NY-CO-7 | SDCCAG7 | STUB1 | UBOX1 | CLL-associated antigen KW-8
UniProt Number
Q9UNE7
NCBI GenBank
NM_005861 | NP_005852.2
SwissProt
Q9UNE7

Target

Target
Human STUB1 / CHIP
Antigen Species
Human
Epitope
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner (PubMed:23973223).
Gene Name
STUB1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant Human E3 ubiquitin-protein ligase CHIP protein (1-48AA)
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Concentration
3 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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