LSBio
Polyclonal Rabbit anti-Human SMAD5 Antibody (IHC, IF, WB)

1:100 staining mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Polyclonal Rabbit anti-Human SMAD5 Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human SMAD5 antibodies →
$288.00each
Catalog Number: LS-C801270-100

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Overview

Description
SMAD5 antibody LS-C801270 is an unconjugated rabbit polyclonal antibody to SMAD5 from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
JV5-1 | MADH5 | SMAD5 | SMAD 5 | MAD homolog 5 | Mothers against DPP homolog 5 | SMA- and MAD-related protein 5 | SMAD family member 5 | DWFC | HSmad5
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q99717
SwissProt
Q99717

Target

Target
Human SMAD5
Antigen Species
Human
Epitope
Smad5 Antibody detects endogenous levels of total Smad5.
Gene Name
SMAD5

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Synthesized peptide derived from human Smad5.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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