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Polyclonal Rabbit anti-Human SH3BP2 Antibody (phospho-Ser427, WB)

Anti-SH3BP2 pS427 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates). The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kD corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.

Polyclonal Rabbit anti-Human SH3BP2 Antibody (phospho-Ser427, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$579.00each
Catalog Number: LS-C18896-100

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Overview

Description
SH3BP2 antibody LS-C18896 is an unconjugated rabbit polyclonal antibody to SH3BP2 (pSer427) from human. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Abl-SH3 binding protein 2 | 3BP-2 | 3BP2 | Cherubism | CRPM | RES4-23 | SH3 domain-binding protein 2 | SH3BP2 | TNFAIP3 interacting protein 2 | CRBM | SH3-domain binding protein 2
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P78314
NCBI GenBank
NM_003023 | NP_003014.3
SwissProt
P78314

Target

Target
Human SH3BP2
Antigen Species
Human
Gene Name
SH3BP2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP3 protein (SH3 Domain Binding Protein 2).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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