LSBio
Polyclonal Rabbit anti-Human SFN / Stratifin / 14-3-3 Sigma Antibody (Preservative Free, IF, WB)

SFN was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 2 uL anti-SFN rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-SFN rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 32 kDa.

Polyclonal Rabbit anti-Human SFN / Stratifin / 14-3-3 Sigma Antibody (Preservative Free, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human SFN / Stratifin / 14-3-3 Sigma antibodies →
$309.00each
Catalog Number: LS-C817744-100

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Overview

Description
14-3-3 Sigma antibody LS-C817744 is an unconjugated rabbit polyclonal antibody to human 14-3-3 Sigma (SFN / Stratifin). Validated for ELISA, ICC, IF, IP and WB.
Application
ELISA, ICC, IF, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
14-3-3 sigma | 14-3-3 protein sigma | HME1 | SFN | Stratifin | YWHAS
Recommended Dilution: ELISA
1:5000 - 1:10000
Recommended Dilution: IF/ICC
1:300 - 1:10000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P31947
NCBI GenBank
NM_006142 | NP_006133.1
SwissProt
P31947

Target

Target
Human SFN / Stratifin / 14-3-3 Sigma
Antigen Species
Human
Epitope
Human 14-3-3 sigma
Gene Name
SFN

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant Human 14-3-3 sigma / Stratifin / YWHAS Protein
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.2 µm filtered solution in PBS
Volume
100 Microliter
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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