LSBio
Polyclonal Rabbit anti-Human RFX5 Antibody (WB)

Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.

Polyclonal Rabbit anti-Human RFX5 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Chromatin Immunoprecipitation, Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Immunoprecipitation, Other, Western Blot
Reactivity:Human
Format:Liquid
See all Human RFX5 antibodies →
$579.00each
Catalog Number: LS-C18752-100

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Overview

Description
RFX5 antibody LS-C18752 is an unconjugated rabbit polyclonal antibody to human RFX5. Validated for ChrIP, ELISA, GS and WB.
Application
ChIP, ELISA, IB, IHC, IP, Other, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
RFX5 | Binding regulatory factor | DNA-binding protein RFX5 | Regulatory factor X 5
Recommended Dilution: ELISA
1:5000 - 1:25000
Recommended Dilution: WB
1:1000
UniProt Number
P48382
NCBI GenBank
NM_000449 | NP_000440.1
SwissProt
P48382

Target

Target
Human RFX5
Antigen Species
Human
Gene Name
RFX5

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.