Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-p65 NLS Antibody - Immunohistochemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells. Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody. Cultured cells shown above were formalin-fixed. tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval. Blocking and hybridization included 5% NGS.
Polyclonal Rabbit anti-Human RELA / NFKB p65 Antibody (IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Other, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18905-100
Catalog Number: LS-C18905
For bulk orders or custom formulations:
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- Description
- NFKB p65 antibody LS-C18905 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA). Validated for ELISA, GS, IF, IHC and WB. Cited in 3 publications.
- Application
- ELISA, IB, IF, IHC, IHC-P, Other, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
- Recommended Dilution: ELISA
- 1:5000 - 1:25000
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC-P
- 1:200
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- Q04206
- NCBI GenBank
- NM_021975 | NP_068810.3
- SwissProt
- Q04206
Target
- Target
- Human RELA / NFKB p65
- Antigen Species
- Human
- Epitope
- The antibody selectively binds to the activated form of NFkB. Anti-NFkB p65 NLS may react non-specifically with other proteins.
- Gene Name
- RELA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex. Starkey JM, Haidacher SJ, LeJeune WS, Zhang X, Tieu BC, Choudhary S, Brasier AR, Denner LA, Tilton RG. Diabetes. 2006;55:1252-9.
PubMed: 16644679
Antiinflammatory adaptation to hypoxia through adenosine-mediated cullin-1 deneddylation. Khoury J, Ibla JC, Neish AS, Colgan SP. The Journal of clinical investigation. 2007;117:703-11.
PubMed: 17318263 · PMC: PMC1797604
Adenosine signaling mediates SUMO-1 modification of IkappaBalpha during hypoxia and reoxygenation. Liu Q, Li J, Khoury J, Colgan SP, Ibla JC. The Journal of biological chemistry. 2009;284:13686-95.
PubMed: 19297320 · PMC: PMC2679470
