LSBio
Polyclonal Rabbit anti-Human PRKCE / PKC-Epsilon Antibody (phospho-Ser729, WB)

Western blot analysis of extracts from HeLa cells (line 1), Jurkat cells (line 2) and NIH/3T3 cells (line 3) all treated with PMA (125ng/ml, 30mins), using PKC e (Phospho-Ser729) antibody.

Polyclonal Rabbit anti-Human PRKCE / PKC-Epsilon Antibody (phospho-Ser729, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human PRKCE / PKC-Epsilon antibodies →
$365.00each
Catalog Number: LS-C822150-100

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Overview

Description
PKC-Epsilon antibody LS-C822150 is an unconjugated rabbit polyclonal antibody to PKC-Epsilon (PRKCE) (pSer729) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
NPKC-epsilon | PKC epsilon | Protein kinase C epsilon | Protein kinase C epsilon type | Pkcepsilon | PKCE | PRKCE | Protein kinase C, epsilon
Recommended Dilution: ELISA
1:10000
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q02156
SwissProt
Q02156

Target

Target
Human PRKCE / PKC-Epsilon
Antigen Species
Human
Epitope
PKC Epsilon (Phospho-Ser729) antibody detects endogenous levels of PKC Epsilon only when phosphorylated at serine 729. (Human: Ser729; Mouse: Ser729; Rat: Ser729)
Gene Name
PRKCE

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Synthesized phosphopeptide derived from human PKC Epsilon around the phosphorylation site of serine 729 (G-F-SP-Y-F).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year.
Recommended Storage Buffer
PBS|150mM NaCl

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