LSBio
Polyclonal Rabbit anti-Human PRAME Antibody (WB)

Western blot analysis of extracts of various cell lines, using PRAME antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.

Polyclonal Rabbit anti-Human PRAME Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human PRAME antibodies →
$281.00each
Catalog Number: LS-C749507-20

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Overview

Description
PRAME antibody LS-C749507 is an unconjugated rabbit polyclonal antibody to PRAME from human. It is reactive with human, mouse and rat. Validated for WB.
Application
WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Cancer/testis antigen 130 | CT130 | MAPE | Opa-interacting protein 4 | Opa-interacting protein OIP4 | PRAME | OIP-4 | OIP4
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P78395
NCBI GenBank
NM_006115 | NP_006106.1
SwissProt
P78395

Target

Target
Human PRAME
Antigen Species
Human
Gene Name
PRAME

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1). MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVK
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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