Western blot analysis of extracts of various cell lines, using Phospho-PPP1R12A-S507 antibody at 1:2000 dilution or PPP1R12A antibody. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.