LSBio
Polyclonal Rabbit anti-Human POLR1B Antibody (WB)

Western blot analysis of extracts of various cell lines, using POLR1B antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.

Polyclonal Rabbit anti-Human POLR1B Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human POLR1B antibodies →
$281.00each
Catalog Number: LS-C747242-20

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Overview

Description
POLR1B antibody LS-C747242 is an unconjugated rabbit polyclonal antibody to POLR1B from human. It is reactive with human and mouse. Validated for WB.
Application
WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
POLR1B | RNA polymerase I subunit 2 | RPA135 | Rpo1-2
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q9H9Y6
SwissProt
Q9H9Y6

Target

Target
Human POLR1B
Antigen Species
Human
Gene Name
POLR1B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2). IANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAE
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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