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Polyclonal Rabbit anti-Human PNP / Nucleoside Phosphorylase Antibody (IHC, WB)

Flow Cytometry analysis of U937 cells using anti-PNP antibody. Overlay histogram showing U937 cells stained with anti-PNP antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Polyclonal Rabbit anti-Human PNP / Nucleoside Phosphorylase Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Lyophilized
See all Human PNP / Nucleoside Phosphorylase antibodies →
$480.00each
Catalog Number: LS-C662591-100

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Overview

Description
Nucleoside Phosphorylase antibody LS-C662591 is an unconjugated rabbit polyclonal antibody to human Nucleoside Phosphorylase (PNP). Validated for Flow, ICC, IHC and WB.
Application
FC, ICC, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Inosine phosphorylase | NP | Nucleoside phosphorylase | PNP | PUNP | PRO1837
UniProt Number
P00491
SwissProt
P00491

Target

Target
Human PNP / Nucleoside Phosphorylase
Antigen Species
Human
Epitope
Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology. .
Gene Name
PNP

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PNP (161-189 aa AMSDAYDRTMRQRALSTWKQMGEQRELQE), different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Preservative
Yes

Storage & Handling

Storage Conditions
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Recommended Storage Buffer
NaCl

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