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Polyclonal Rabbit anti-Human PKM / Pyruvate Kinase, Muscle Antibody (aa476-505, IHC, IF, WB)

Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Pyruvate Kinase (PKM2) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).

Polyclonal Rabbit anti-Human PKM / Pyruvate Kinase, Muscle Antibody (aa476-505, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Non-Human Primate, Rat
Format:Liquid
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$401.00each
Catalog Number: LS-C98973-200

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Overview

Description
Pyruvate Kinase, Muscle antibody LS-C98973 is an unconjugated rabbit polyclonal antibody to Pyruvate Kinase, Muscle (PKM) (aa476-505) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and WB.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, NHP, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
p58 | PK3 | PKM | PKM2 | Pyruvate Kinase | Pyruvate kinase 2/3 | Pyruvate kinase, m1 | Pyruvate kinase, muscle | TCB | Tumor M2-PK | OIP-3 | OIP3 | Opa-interacting protein 3 | CTHBP | PK, muscle type | PK2 | Pyruvate kinase isozymes M1/M2 | Pyruvate kinase muscle isozyme | THBP1
Recommended Dilution: IF/ICC
1:200
Recommended Dilution: WB
1:1000
UniProt Number
P14618
NCBI GenBank
NM_002654 | NP_002645.3
SwissProt
P14618

Target

Target
Human PKM / Pyruvate Kinase, Muscle
Antigen Species
Human
Epitope
This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
Gene Name
PKM

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
200 Microliter
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS

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