Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Polyclonal Rabbit anti-Human PDPK1 / PDK1 Antibody (C-Terminus, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18965-100
Catalog Number: LS-C18965
For bulk orders or custom formulations:
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- Description
- PDK1 antibody LS-C18965 is an unconjugated rabbit polyclonal antibody to human PDK1 (PDPK1) (C-Terminus). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Dog, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HPDK1 | PkB kinase like gene 1 | PkB-like 1 | Pdk-1 | PDPK1 | PkB kinase | PRO0461
- Recommended Dilution: ELISA
- 1:100000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O15530
- NCBI GenBank
- NM_002613 | NP_002604.1
- SwissProt
- O15530
Target
- Target
- Human PDPK1 / PDK1
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest cross-reactivity with PDK-1 protein from mouse, rat and dog based on 92% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown.
- Gene Name
- PDPK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human PDK-1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.3 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
