LSBio
Polyclonal Rabbit anti-Human NUP35 / NUP53 Antibody (WB)

Western blot analysis of extracts of various cell lines, using NUP35 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Polyclonal Rabbit anti-Human NUP35 / NUP53 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human NUP35 / NUP53 antibodies →
$281.00each
Catalog Number: LS-C747839-20

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Overview

Description
NUP53 antibody LS-C747839 is an unconjugated rabbit polyclonal antibody to NUP53 (NUP35) from human. It is reactive with human and mouse. Validated for WB.
Application
WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
35 kDa nucleoporin | Mitotic phosphoprotein 44 | MP-44 | Nucleoporin Nup35 | Nucleoporin NUP53 | Nucleoporin 35kDa | MP44 | NP44 | NUP35 | NUP53
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q8NFH5
NCBI GenBank
AA160569
SwissProt
Q8NFH5

Target

Target
Human NUP35 / NUP53
Antigen Species
Human
Gene Name
NUP35

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human NUP35 (NP_612142.2). MAAFAVEPQGPALGSEPMMLGSPTSPKPGVNAQFLPGFLMGDLPAPVTPQPRSISGPSVGVMEMRSPLLAGGSPPQPVVPAHKDKSGAPPVRSIYDDISSPGLGSTPLTSRRQPNISVMQSPLVGVTSTPGTGQSMFSPASIGQPRKTTLSPAQLDPFYTQGDSLTSEDHLDDSWVTVFGFPQASASYILLQFAQYGNILKHVMSNTGNWMHIRYQSKLQARKALSKDGRIFGESIMIGVKPCIDKSVMESSDRCALSSPSLAFTPPIKTLGTPTQPGSTPRISTMRPLATAYKASTSDYQVISDRQTPKKDESLVSKAMEYMFGW
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.