Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western Blot of Rabbit anti-Myosin-6 antibody. Lane 1: SF9 cell lysate of truncated smooth myosin. Lane 2: Jurkat lysate. Lane 3: LnCap lysate. Lane 4: Recombinant myosin VI Load: 35 ug per lane for cell lysate. 50 ng of recombinant protein. Primary antibody: Myosin 6 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 150 kDa for Myo6.
Polyclonal Rabbit anti-Human Myosin VI / MYO6 Antibody (WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Pig |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209179-100
Catalog Number: LS-C209179
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- MYO6 antibody LS-C209179 is an unconjugated rabbit polyclonal antibody to MYO6 (Myosin VI) from human. It is reactive with human and pig. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Por
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DFNB37 | DFNA22 | MYO6 | Myosin VI | Myosin-VI | Unconventional myosin-VI | Unconventional myosin-6 | KIAA0389
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9UM54
- SwissProt
- Q9UM54
Target
- Target
- Human Myosin VI / MYO6
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with 94% human Myosin VI. Cross-reactivity with myosin6 from other sources have not been determined.
- Gene Name
- MYO6
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 25 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
