LSBio
Polyclonal Rabbit anti-Human MAPK3 / ERK1 Antibody (WB)

Western blot analysis of Mouse, Rat Brain showing detection of ~43.1, 38.2, 40.1 kDa ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~43.1, 38.2, 40.1 kDa. Other Band(s): 50 kDa.

Polyclonal Rabbit anti-Human MAPK3 / ERK1 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human MAPK3 / ERK1 antibodies →
$451.00each
Catalog Number: LS-C776543-100

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Overview

Description
ERK1 antibody LS-C776543 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) from human. It is reactive with human, mouse and rat. Validated for WB.
Application
IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | MAPK3 | PRKM3
Recommended Dilution: WB
1:1000
UniProt Number
P27361
NCBI GenBank
CAA42744.1, X60188
SwissProt
P27361

Target

Target
Human MAPK3 / ERK1
Antigen Species
Hu
Epitope
Detects 50 kDa. Band is higher than the predicted molecular weight due to post translational modifications.
Gene Name
MAPK3

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
Synthetic peptide from the C-Terminus of Human ERK1 (aa. 270-379)
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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