LSBio
Polyclonal Rabbit anti-Human MAPK1 / ERK2 Antibody (phospho-Thr180/Tyr182, IF, WB)

Polyclonal Rabbit anti-Human MAPK1 / ERK2 Antibody (phospho-Thr180/Tyr182, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human MAPK1 / ERK2 antibodies →
$732.00each
Catalog Number: LS-C16326-200

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Overview

Description
ERK2 antibody LS-C16326 is an unconjugated rabbit polyclonal antibody to human ERK2 (MAPK1) (pThr180/Tyr182). Validated for ELISA, Flow, ICC, IF, IP and WB.
Application
ELISA, FC, ICC, IF, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | MAPK1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Recommended Dilution: FC
1:100
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: IP
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P28482
SwissProt
P28482

Target

Target
Human MAPK1 / ERK2
Antigen Species
Human
Epitope
Detects endogenous levels of p38 MAP kinase only when activated by dual phosphorylation at Thr180 and Tyr182. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182. Does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species cross-reactivity: Human.
Gene Name
MAPK1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 50% Glycerol, BSA
Volume
200 Microliter
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

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