LSBio
Polyclonal Rabbit anti-Human MAP2K4 / MKK4 Antibody (IF, WB)

Positive WB detected in:Hela whole cell lysate,MCF-7 whole cell lysate;All lanes: MAP2K4 antibody at 3.25ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 45,46 kDa;Observed band size: 45 kDa;

Polyclonal Rabbit anti-Human MAP2K4 / MKK4 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Human
Format:Liquid
See all Human MAP2K4 / MKK4 antibodies →
$300.00each
Catalog Number: LS-C499279-50

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Overview

Description
MKK4 antibody LS-C499279 is an unconjugated rabbit polyclonal antibody to human MKK4 (MAP2K4). Validated for ELISA, IF and WB.
Application
ELISA, IF, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
JNK activating kinase 1 | JNK-activated kinase 1 | JNK-activating kinase 1 | MAPK/ERK kinase 4 | MAPKK4 | JNKK1 | PRKMK4 | SAPKK1 | SEK1 | MEK4 | MKK4 | SAPK/ERK kinase 1 | SERK1 | JNKK | MAP kinase kinase 4 | MAP2K4 | MAPKK 4 | MEK 4 | SAPK kinase 1 | SAPKK-1 | SKK1
UniProt Number
P45985
NCBI GenBank
NM_003010, NP_003001.1
SwissProt
P45985

Target

Target
Human MAP2K4 / MKK4
Antigen Species
Hu
Epitope
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
Gene Name
MAP2K4

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant Human Dual specificity mitogen-activated protein kinase kinase 4 protein (259-398AA)
Immunogen Type
Recom

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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