Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
Polyclonal Rabbit anti-Human MAP2K2 / MKK2 / MEK2 Antibody (C-Terminus, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745391-100
Catalog Number: LS-C745391
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- Description
- MEK2 antibody LS-C745391 is an unconjugated rabbit polyclonal antibody to MEK2 (MAP2K2 / MKK2) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Predicted Reactivity
- Human, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK activator kinase 2 | MAP kinase kinase 2 | MAPK/ERK kinase 2 | MAPKK 2 | MKK2 | MEK 2 | MEK2 | MAP2K2 | MAPKK2 | PRKMK2
- Recommended Dilution: ELISA
- 5 ug/ml
- Recommended Dilution: IHC
- 1:100
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P36507
- NCBI GenBank
- NM_030662 | NP_109587.1
- SwissProt
- P36507
Target
- Target
- Human MAP2K2 / MKK2 / MEK2
- Antigen Species
- Human
- Epitope
- Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
- Gene Name
- MAP2K2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-MEK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-Terminus conjugated to KLH.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.09 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
