LSBio
Polyclonal Rabbit anti-Human LY9 / CD229 Antibody (WB)

Western blot analysis of extracts of various cell lines, using LY9 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Polyclonal Rabbit anti-Human LY9 / CD229 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human LY9 / CD229 antibodies →
$281.00each
Catalog Number: LS-C496743-20

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Overview

Description
CD229 antibody LS-C496743 is an unconjugated rabbit polyclonal antibody to CD229 (LY9) from human. It is reactive with human and mouse. Validated for WB.
Application
IHC, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD229 | CD229 antigen | Cell surface molecule Ly-9 | Hly9 | Lymphocyte antigen 9 | MLY9 | SLAMF3 | LY9
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q9HBG7
SwissProt
Q9HBG7

Target

Target
Human LY9 / CD229
Antigen Species
Human
Gene Name
LY9

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 160-360 of human LY9 (NP_001248386.1). TMKSVKVSENFSCNITLMCSVKGAEKSVLYSWTPREPHASESNGGSILTVSRTPCDPDLPYICTAQNPVSQRSSLPVHVGQFCTDPGASRGGTTGETVVGVLGEPVTLPLALPACRDTEKVVWLFNTSIISKEREEAATADPLIKSRDPYKNRVWVSSQDCSLKISQLKIEDAGPYHAYVCSEASSVTSMTHVTLLIYRPE
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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