LSBio
Polyclonal Rabbit anti-Human LIG4 / DNA Ligase IV Antibody (IHC, WB)

Western blot analysis of extracts of various cell lines, using LIG4 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Polyclonal Rabbit anti-Human LIG4 / DNA Ligase IV Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human LIG4 / DNA Ligase IV antibodies →
$281.00each
Catalog Number: LS-C331665-20

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Overview

Description
DNA Ligase IV antibody LS-C331665 is an unconjugated rabbit polyclonal antibody to DNA Ligase IV (LIG4) from human. It is reactive with human and mouse. Validated for IHC and WB.
Application
IHC, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DNA ligase IV | DNA joinase | DNA ligase 4 | DNA repair enzyme | Ligase IV, DNA, ATP-dependent | LIG4 | Sealase | Polynucleotide ligase
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P49917
NCBI GenBank
NM_002312 | NP_002303.2
SwissProt
P49917

Target

Target
Human LIG4 / DNA Ligase IV
Antigen Species
Human
Epitope
Human LIG4 / DNA Ligase IV
Gene Name
LIG4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human LIG4 (NP_002303.2). SKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.