LSBio
Polyclonal Rabbit anti-Human KDR / VEGFR2 / FLK1 Antibody (phospho-Tyr996, IHC, WB)

Anti-Phospho-KDR/FLK1-Y996 antibody is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate. Endothelial cells were stimulated with 50 ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody diluted to 1:500 in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.

Polyclonal Rabbit anti-Human KDR / VEGFR2 / FLK1 Antibody (phospho-Tyr996, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human KDR / VEGFR2 / FLK1 antibodies →
$401.00each
Catalog Number: LS-C96876-400

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Overview

Description
FLK1 antibody LS-C96876 is an unconjugated rabbit polyclonal antibody to human FLK1 (KDR / VEGFR2) (pTyr996). Validated for IHC and WB.
Application
IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | KDR | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
Recommended Dilution: IHC-P
1:50 - 1:100
Recommended Dilution: WB
1:1000
UniProt Number
P35968
SwissProt
P35968

Target

Target
Human KDR / VEGFR2 / FLK1
Antigen Species
Human
Epitope
This KDR/FLK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y996 of human KDR/FLK1.
Gene Name
KDR

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
400 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS

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