LSBio
Polyclonal Rabbit anti-Human ISL2 / Islet 2 Antibody (aa1-30, IF, WB)

Fluorescent image of HUVEC cell stained with ISL2 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). ISL2 immunoreactivity is localized to Nucleus significantly.

Polyclonal Rabbit anti-Human ISL2 / Islet 2 Antibody (aa1-30, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human ISL2 / Islet 2 antibodies →
$401.00each
Catalog Number: LS-C165303-400

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Overview

Description
Islet 2 antibody LS-C165303 is an unconjugated rabbit polyclonal antibody to human Islet 2 (ISL2) (aa1-30). Validated for IF and WB. Cited in 1 publication.
Application
IF, IHC, WB
Reactivity
Hu
Predicted Reactivity
Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ISL2 | ISL LIM homeobox 2 | Islet-2
Recommended Dilution: IF/ICC
1:10 - 1:50
Recommended Dilution: WB
1:1000
UniProt Number
Q96A47
SwissProt
Q96A47

Target

Target
Human ISL2 / Islet 2
Antigen Species
Human
Epitope
This ISL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ISL2.
Gene Name
ISL2

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
400 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS
A molecular mechanism for the topographic alignment of convergent neural maps. Savier E, Eglen SJ, Bathélémy A, Perraut M, Pfrieger FW, Lemke G, Reber M. eLife. 2017 March;6:pii: e20470. PubMed: 28322188 PMC: PMC5360444

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