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Polyclonal Rabbit anti-Human INPPL1 / SHIP2 Antibody (IHC, WB)

IF analysis of INPPL1 using anti-INPPL1 antibody INPPL1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-INPPL1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Polyclonal Rabbit anti-Human INPPL1 / SHIP2 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
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$480.00each
Catalog Number: LS-C756515-100

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Overview

Description
SHIP2 antibody LS-C756515 is an unconjugated rabbit polyclonal antibody to SHIP2 (INPPL1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Application
ELISA, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
51C protein | INPPL-1 | INPPL1 | SHIP-2 | Protein 51C | SHIP2
Recommended Dilution: ELISA
0.1 - 0.5 ug/ml
Recommended Dilution: IHC-P
0.5 - 1 µg/ml
Recommended Dilution: WB
0.1 - 0.5 µg/ml
UniProt Number
O15357
SwissProt
O15357

Target

Target
Human INPPL1 / SHIP2
Antigen Species
Human
Epitope
No cross reactivity with other proteins.
Gene Name
INPPL1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
E. coli-derived human INPPL1 recombinant protein (Position: R1172-K1258).
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer
NaCl

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