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Polyclonal Rabbit anti-Human HSF1 Antibody (N-Terminus, WB)

Western Blot of rabbit anti-HSF1 antibody. Lane 1: Ladder (Opal PreStained p/n MB-210-0500). Lane 2: A431 Whole Cell lysate p/n W09-000-361. Load: 35 µg per lane. Primary antibody: HSF1 antibody at 1.0 ug/ml for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Block: Univeral Buffer BlockOut (MB-073) for 30 min at room temperature. Predicted/Observed size: ~57kDa for HSF1 in A431 whole cell lysate.

Polyclonal Rabbit anti-Human HSF1 Antibody (N-Terminus, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human HSF1 antibodies →
$579.00each
Catalog Number: LS-C745412-100

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Overview

Description
HSF1 antibody LS-C745412 is an unconjugated rabbit polyclonal antibody to human HSF1 (N-Terminus). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HSF1 | HSTF 1 | Heat shock factor protein 1 | HSF 1 | HSTF1
Recommended Dilution: ELISA
1:50000
Recommended Dilution: WB
1 µg/ml
UniProt Number
Q00613
SwissProt
Q00613

Target

Target
Human HSF1
Antigen Species
Hu
Epitope
A BLAST analysis was used to suggest reactivity with this protein in mouse and bovine species based on 100% homology for the immunogen sequence.
Gene Name
HSF1

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Affinity purified Anti-HSF1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the n-terminal portion of human HSF1 protein.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.96 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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