LSBio
Polyclonal Rabbit anti-Human HLA-DRB5 Antibody (WB)

Western blot analysis of extracts of various cell lines, using HLA-DRB5 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.

Polyclonal Rabbit anti-Human HLA-DRB5 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human
Format:Liquid
See all Human HLA-DRB5 antibodies →
$281.00each
Catalog Number: LS-C747812-20

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Overview

Description
HLA-DRB5 antibody LS-C747812 is an unconjugated rabbit polyclonal antibody to human HLA-DRB5. Validated for WB.
Application
WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DR-9 | DR beta-5 | Dw2 | HLA-DRB | Human leucocyte antigen DRB5 | HLA-DRB5 | MHC class II antigen DRB1*9 | MHC class II HLA beta chain | MHC HLA-DR-beta chain | Leukocyte antigen class II | DR2-beta-2 | DR9 | MHC class II antigen DRB5
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
Q30154
NCBI GenBank
NM_002125 | NP_002116.2
SwissProt
Q30154

Target

Target
Human HLA-DRB5
Antigen Species
Human
Gene Name
HLA-DRB5

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB5 (NP_002116.2). GDTRPRFLQQDKYECHFFNGTERVRFLHRDIYNQEEDLRFDSDVGEYRAVTELGRPDAEYWNSQKDFLEDRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPARTQTLQHHNLLVCSVNGFYPGSIEVRWFRNSQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRAQSESAQSK
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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