LSBio
Polyclonal Rabbit anti-Human Histone H4 Antibody (N-Terminus, WB)

Western Blot of rabbit anti-Histone H4 antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: HeLa Nuclear Extract. Lane 3: HeLa Whole Cell Lysate. Lane 4: HeLa Nuclear Extract. Load: 15 µg per lane. Primary antibody: Lanes 1-2: Histone H3 K18Ac, Lanes 3-4: Histone H4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~15.4kDa for Histone H3, and 11.3kDa for Histone H4.

Polyclonal Rabbit anti-Human Histone H4 Antibody (N-Terminus, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human Histone H4 antibodies →
$579.00each
Catalog Number: LS-C744602-100

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Overview

Description
Histone H4 antibody LS-C744602 is an unconjugated rabbit polyclonal antibody to human Histone H4 (N-Terminus). Validated for DB, ELISA and WB.
Application
DB, ELISA, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:20000
Recommended Dilution: WB
1:500 - 1:2000

Target

Target
Human Histone H4
Antigen Species
Hu
Epitope
Cross reactivity with other species was not tested.

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminal region of histone H4.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
90 mg/mL
Volume
100 Microliter
Preservative
Yes

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.