LSBio
Polyclonal Rabbit anti-Human Histone H4 Antibody (IHC, IF, WB)

Western blot analysis of extracts of HeLa cells, using HIST4H4 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Polyclonal Rabbit anti-Human Histone H4 Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human, Rat
Format:Liquid
See all Human Histone H4 antibodies →
$281.00each
Catalog Number: LS-C346056-20

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Overview

Description
Histone H4 antibody LS-C346056 is an unconjugated rabbit polyclonal antibody to Histone H4 from human. It is reactive with human and rat. Validated for IF, IHC, IP and WB.
Application
IF, IHC, IP, WB
Reactivity
Hu, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: IF/ICC
1:20 - 1:100
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: IP
1:20 - 1:100
Recommended Dilution: WB
1:500 - 1:2000

Target

Target
Human Histone H4
Antigen Species
Human
Epitope
Human Histone H4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human Histone H4 (NP_001029249.1). MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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