LSBio
Polyclonal Rabbit anti-Human Histone H3 Antibody (WB)

Dot Blot of Rabbit Histone H3 K27 me1-3 ac Antibody. Lane 1: Unmodified. Lane 2: me1. Lane 3: me2. Lane 4: me3. Lane 5: ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [1-3 methyl, Lys27, ac] antibody at 0.5ug/ml for 45 min at 4 degrees C. Secondary antibody: Dylight alpha 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C.

Polyclonal Rabbit anti-Human Histone H3 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Helminth / Nematode, Human, Mouse, Rat
Format:Liquid
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$579.00each
Catalog Number: LS-C209233-50

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Overview

Description
Histone H3 antibody LS-C209233 is an unconjugated rabbit polyclonal antibody to Histone H3 from human. It is reactive with human, mouse, rat and other species. Validated for DB, ELISA and WB.
Application
DB, ELISA, WB
Reactivity
Helm, Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: WB
1:1000

Target

Target
Human Histone H3
Antigen Species
Human

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Histone H3 K27 methyl Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27 me3 site of human Histone H3.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.99 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.