LSBio
Polyclonal Rabbit anti-Human HIST4H4 Antibody (Ac-Lys12, IHC, IF, WB)

Dot Blot of rabbit Histone H4 K12-Ac (RABBIT) Antibody. Lane 1: K12 unmodified. Lane 2: K12-Acetylated. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 K12-Ac antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody HRP Conjugated at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.

Polyclonal Rabbit anti-Human HIST4H4 Antibody (Ac-Lys12, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human HIST4H4 antibodies →
$594.00each
Catalog Number: LS-C745499-50

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Overview

Description
HIST4H4 antibody LS-C745499 is an unconjugated rabbit polyclonal antibody to human HIST4H4 (Ac-Lys12). Validated for DB, IF, IHC and WB.
Application
DB, IF, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HIST2H4 | Histone 4, H4 | Histone cluster 4, H4 | HIST4H4 | Histone H4
Recommended Dilution: IF/ICC
1:10
Recommended Dilution: IHC
1:100
Recommended Dilution: WB
1 µg/ml
NCBI GenBank
NM_175054 | NP_778224.1

Target

Target
Human HIST4H4
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest cross-reactivity with Alfalfa based on 100% sequence homology. Cross-reactivity with Histone H4 [ac Lys12] from other sources has not been determined.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 12 of human Histone H4.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.69 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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