LSBio
Polyclonal Rabbit anti-Human HFE Antibody (IHC, IF, WB)

Western blot analysis of extracts of various cell lines, using HFE antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Polyclonal Rabbit anti-Human HFE Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human HFE antibodies →
$281.00each
Catalog Number: LS-C331410-20

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Overview

Description
HFE antibody LS-C331410 is an unconjugated rabbit polyclonal antibody to HFE from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
Application
IF, IHC, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HFE1 | HH | MHC class I-like protein HFE | Hemochromatosis | HFE | High Fe | MVCD7 | HLA-H | HLAH | TFQTL2
Recommended Dilution: IF/ICC
1:50 - 1:200
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q30201
SwissProt
Q30201

Target

Target
Human HFE
Antigen Species
Human
Epitope
Human HFE
Gene Name
HFE

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-306 of human HFE (NP_000401.1). SSQMWLQLSQSLKGWDHMFTVDFWTIMENHNHSKESHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQVPPLVKVTHHVTSSVTTLRCRALNYYPQNITMKWLKDKQPMDAKEFEPKDVLPNGDGTYQGWITLAVPPGEEQRYTCQVEHPGLDQPLIVIWEPSPSGTLV
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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