LSBio
Polyclonal Rabbit anti-Human GOLPH3 Antibody (IHC, WB)

Flow Cytometry analysis of U87 cells using anti-GOLPH3 antibody. Overlay histogram showing U87 cells stained with anti-GOLPH3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GOLPH3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Polyclonal Rabbit anti-Human GOLPH3 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
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$480.00each
Catalog Number: LS-C756473-100

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Overview

Description
GOLPH3 antibody LS-C756473 is an unconjugated rabbit polyclonal antibody to GOLPH3 from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Application
ELISA, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Coat protein GPP34 | Coat-protein | Golgi protein | Golgi phosphoprotein 3 | GPP34 | Golgi-associated protein | GOPP1 | MIDAS | GOLPH3
Recommended Dilution: ELISA
0.1 - 0.5 ug/ml
Recommended Dilution: IHC-P
0.5 - 1 µg/ml
Recommended Dilution: WB
0.1 - 0.5 µg/ml
UniProt Number
Q9H4A6
SwissProt
Q9H4A6

Target

Target
Human GOLPH3
Antigen Species
Human
Epitope
No cross reactivity with other proteins.
Gene Name
GOLPH3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
E. coli-derived human GOLPH3 recombinant protein (Position: K56-K298).
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer
NaCl

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