LSBio
Polyclonal Rabbit anti-Human GLI2 Antibody (IHC, WB)

Western blot using the affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 µg of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results.

Polyclonal Rabbit anti-Human GLI2 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
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$311.00each
Catalog Number: LS-C745295-25

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Overview

Description
GLI2 antibody LS-C745295 is an unconjugated rabbit polyclonal antibody to GLI2 from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Predicted Reactivity
Non-Human Primate
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GLI2 | GLI-Kruppel family member GLI2 | HPE9 | Gli-2 | Oncogene GLI2 | Tax helper protein 2 | THP | Zinc finger protein GLI2 | Tax helper protein | THP2 | GLI family zinc finger 2 | Tax helper protein 1 | THP1
Recommended Dilution: ELISA
1:2000 - 1:12000
Recommended Dilution: IHC
1:500 - 1:2000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P10070
NCBI GenBank
NM_005270 | NP_005261.2
SwissProt
P10070

Target

Target
Human GLI2
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.
Gene Name
GLI2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.