Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Fluorescent image of U251 cells stained with GABARAP antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated GABARAP primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Polyclonal Rabbit anti-Human GABARAP Antibody (aa1-30, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C166311-400
Catalog Number: LS-C166311
For bulk orders or custom formulations:
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- Description
- GABARAP antibody LS-C166311 is an unconjugated rabbit polyclonal antibody to human GABARAP (aa1-30). Validated for IF, IHC and WB. Cited in 1 publication.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Mouse, Rabbit, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATG8A | MM46 | FLC3B | GABARAP | GABARAP-a
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: IHC-P
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O95166
- NCBI GenBank
- NM_007278 | NP_009209.1
- SwissProt
- O95166
Target
- Target
- Human GABARAP
- Antigen Species
- Human
- Epitope
- This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GABARAP.
- Gene Name
- GABARAP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Induction of autophagy by Imatinib sequesters Bcr-Abl in autophagosomes and down-regulates Bcr-Abl protein. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. American journal of hematology. 2013. PubMed: 23440701
