Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-HER3 Antibody - Immunoprecipitation/Western Blot. Combined immunoprecipitation and western blot of a human cell lysate using anti-HER3 antibody. Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-b1 for 15 at 100 ng/ml and without (-) the addition of Heregulin-b1. The combination of immunoprecipitation and western blotting was performing using 5 ul of the anti-HER3 antibody for immunoprecipitation followed by western blot detection using an anti-phosphotyrosine antibody. Visualization occurred using an ECL system. Film exposure approximately 1. Other detection systems will yield similar results.
Polyclonal Rabbit anti-Human ERBB3 / HER3 Antibody (WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$555.00each
Catalog Number: LS-C18797-250
Catalog Number: LS-C18797
For bulk orders or custom formulations:
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- Description
- HER3 antibody LS-C18797 is an unconjugated rabbit polyclonal antibody to HER3 (ERBB3) from human. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-erbB-3 | C-erbB3 | ErbB-3 | ERBB3 | HER3 | p85-sErbB3 | p45-sErbB3 | MDA-BF-1 | ErbB3-S | LCCS2 | p180-ErbB3
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P21860
- NCBI GenBank
- NM_001982 | NP_001973.2
- SwissProt
- P21860
Target
- Target
- Human ERBB3 / HER3
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. The antibody is directed against human HER3 and is useful in determining its presence in immunoprecipitation experiments. This antibody can detect HER3 from human, mouse and rat sources. Reactivity of this antibody with HER3 from other species is unknown.
- Gene Name
- ERBB3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-HER3 whole rabbit serum was prepared by repeated immunizations with a HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 2.5 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
