LSBio
Polyclonal Rabbit anti-Human EIF5B / IF2 Antibody (WB)

Western blot analysis of extracts of various cell lines, using EIF5B antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.

Polyclonal Rabbit anti-Human EIF5B / IF2 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human
Format:Liquid
See all Human EIF5B / IF2 antibodies →
$281.00each
Catalog Number: LS-C750095-20

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Overview

Description
IF2 antibody LS-C750095 is an unconjugated rabbit polyclonal antibody to human IF2 (EIF5B). Validated for WB.
Application
WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
EIF-5B | EIF5B | IF2 | KIAA0741
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
O60841
NCBI GenBank
NM_015904 | NP_056988.3
SwissProt
O60841

Target

Target
Human EIF5B / IF2
Antigen Species
Human
Gene Name
EIF5B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3). MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQ
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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