LSBio
Polyclonal Rabbit anti-Human EIF4A3 Antibody (Biotin)

Polyclonal Rabbit anti-Human EIF4A3 Antibody (Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay
Reactivity:Human
Format:Liquid
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$300.00each
Catalog Number: LS-C498778-50

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Overview

Description
EIF4A3 antibody LS-C498778 is a biotin-conjugated rabbit polyclonal antibody to human EIF4A3. Validated for ELISA.
Application
ELISA
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DDX48 | DEAD box protein 48 | EIF-4A-III | EIF4A-III | EIF4AIII | HNMP 265 | NMP265 | NMP 265 | KIAA0111 | Nuclear matrix protein 265 | EIF4A3 | NUK34
UniProt Number
P38919
NCBI GenBank
NM_014740 | NP_055555.1
SwissProt
P38919

Target

Target
Human EIF4A3
Antigen Species
Human
Epitope
ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.
Gene Name
EIF4A3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant human Eukaryotic initiation factor 4A-III protein (6-166AA)
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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