LSBio
Polyclonal Rabbit anti-Human EGLN3 / PHD3 Antibody (Internal, WB)

Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 use at 1:1000 (A) or 1:5000 (B). Detection: Chemiluminescence. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti-Human EGLN3 / PHD3 Antibody (Internal, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human EGLN3 / PHD3 antibodies →
$394.00each
Catalog Number: LS-C48987-0.1

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Overview

Description
PHD3 antibody LS-C48987 is an unconjugated rabbit polyclonal antibody to PHD3 (EGLN3) (Internal) from human. It is reactive with human, mouse and rat. Validated for WB.
Application
IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HPH-1 | HIF prolyl hydroxylase 3 | HIF-prolyl hydroxylase 3 | HIFPH3 | PHD3 | EGL nine (C.elegans) homolog 3 | Egl nine homolog 3 | EGLN3 | HIF-PH3 | HIFP4H3
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
Q9H6Z9
NCBI GenBank
NM_022073 | NP_071356.1
SwissProt
Q9H6Z9

Target

Target
Human EGLN3 / PHD3
Antigen Species
Human
Epitope
HIF prolyl hydroxylase 3. Tris-citrate/phosphate buffer, pH 7 to 8.
Gene Name
EGLN3

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic peptide corresponding to residues between 50-100 of human Prolyl Hydroxylase Domain-Containing Protein 3 using the numbering given in entry NP_071356.1 (GeneID 112399)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide
Concentration
1 mg/mL
Volume
0.1 Milliliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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