LSBio
Polyclonal Rabbit anti-Human EGLN3 / PHD3 Antibody (IF, WB)

Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod

Polyclonal Rabbit anti-Human EGLN3 / PHD3 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human EGLN3 / PHD3 antibodies →
$288.00each
Catalog Number: LS-C803103-100

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Overview

Description
PHD3 antibody LS-C803103 is an unconjugated rabbit polyclonal antibody to PHD3 (EGLN3) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Application
ELISA, IF, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HPH-1 | HIF prolyl hydroxylase 3 | HIF-prolyl hydroxylase 3 | HIFPH3 | PHD3 | EGL nine (C.elegans) homolog 3 | Egl nine homolog 3 | EGLN3 | HIF-PH3 | HIFP4H3
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q9H6Z9
NCBI GenBank
NM_022073 | NP_071356.1
SwissProt
Q9H6Z9

Target

Target
Human EGLN3 / PHD3
Antigen Species
Human
Epitope
PHD3 Antibody detects endogenous levels of PHD3.
Gene Name
EGLN3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthesized peptide derived from human PHD3.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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