LSBio
Polyclonal Rabbit anti-Human EGF Antibody (WB)

Western blot analysis of extracts of various cell lines, using EGF antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Polyclonal Rabbit anti-Human EGF Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
See all Human EGF antibodies →
$281.00each
Catalog Number: LS-C748658-20

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Overview

Description
EGF antibody LS-C748658 is an unconjugated rabbit polyclonal antibody to EGF from human. It is reactive with human and monkey. Validated for WB.
Application
WB
Reactivity
Hu, NHP
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Beta-urogastrone | HOMG4 | URG | EGF | Epidermal growth factor | Pro-epidermal growth factor
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P01133
NCBI GenBank
NM_001963 | NP_001954.2
SwissProt
P01133

Target

Target
Human EGF
Antigen Species
Human
Gene Name
EGF

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 850-950 of human EGF (NP_001954.2). GEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLI
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.