LSBio
Polyclonal Rabbit anti-Human E2F4 Antibody (IF, WB)

Immunofluorescence staining of E2F4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human E2F4 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Polyclonal Rabbit anti-Human E2F4 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Western Blot
Reactivity:Human
Format:Liquid
See all Human E2F4 antibodies →
$309.00each
Catalog Number: LS-C814802-100

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Overview

Description
E2F4 antibody LS-C814802 is an unconjugated rabbit polyclonal antibody to human E2F4. Validated for ICC, IF and WB.
Application
ICC, IF, WB
Reactivity
Hu
Predicted Reactivity
Mouse
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
E2F-4 | E2F4 | p107/p130-binding protein | Transcription factor E2F4
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q16254
SwissProt
Q16254

Target

Target
Human E2F4
Antigen Species
Human
Epitope
Human E2F4
Gene Name
E2F4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthetic peptide corresponding to the center region of the Human E2F4
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.0, 0.03% Proclin 300
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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