Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Polyclonal Rabbit anti-Human DIAPH2 Antibody (C-Terminus, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C19007-100
Catalog Number: LS-C19007
For bulk orders or custom formulations:
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- Description
- DIAPH2 antibody LS-C19007 is an unconjugated rabbit polyclonal antibody to human DIAPH2 (C-Terminus). Validated for ELISA, IP and WB. Cited in 1 publication.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DRF2 | DIA | DIA2 | DIAPH2 | POF | POF2 | Protein diaphanous homolog 2 | Diaphanous-related formin-2 | Diaphorase-2
- Recommended Dilution: ELISA
- 1:12000 - 1:60000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:1000 - 1:6000
- UniProt Number
- O60879
- NCBI GenBank
- NM_006729 | NP_006720.1
- SwissProt
- O60879
Target
- Target
- Human DIAPH2
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse DIA-2 based on a 93% homology to the immunogen sequence. Cross reactivity with DIA-2 homologues from other sources has not been determined.
- Gene Name
- DIAPH2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.4 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Diaphanous formin mDia2 regulates CENP-A levels at centromeres. Liu C, Mao Y. The Journal of cell biology. 2016;213:415-24. PubMed: 27185834 PMC: PMC4878093
