LSBio
Polyclonal Rabbit anti-Human DDX21 Antibody (IF, WB)

Immunofluorescence staining of DDX21 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DDX21 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.

Polyclonal Rabbit anti-Human DDX21 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human DDX21 antibodies →
$309.00each
Catalog Number: LS-C816230-100

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Overview

Description
DDX21 antibody LS-C816230 is an unconjugated rabbit polyclonal antibody to human DDX21. Validated for ICC, IF, IP and WB.
Application
ICC, IF, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DDX21 | DEAD box protein 21 | Gu protein | GUA | GURDB | Gu-alpha | Nucleolar RNA helicase II | Nucleolar RNA helicase Gu | RH II/Gu | RNA helicase II/Gu alpha | Nucleolar RNA helicase 2 | RH-II/GU | RH-II/GuA
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q9NR30
SwissProt
Q9NR30

Target

Target
Human DDX21
Antigen Species
Human
Epitope
Human DDX21
Gene Name
DDX21

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
E. coli-derived Human DDX21 fragment
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.0, 0.03% Proclin 300
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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