LSBio
Polyclonal Rabbit anti-Human CX3CL1 / Fractalkine Antibody (WB)

Western Blot of rabbit anti-Fractalkine antibody. Lane 1: Human Fractalkine Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fractalkine antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 9 kDa, 9 kDa for Human Fractalkine. Other band(s): None.

Polyclonal Rabbit anti-Human CX3CL1 / Fractalkine Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human CX3CL1 / Fractalkine antibodies →
$311.00each
Catalog Number: LS-C745244-25

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Overview

Description
Fractalkine antibody LS-C745244 is an unconjugated rabbit polyclonal antibody to human Fractalkine (CX3CL1). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ABCD-3 | C-X3-C motif chemokine 1 | C3Xkine | Chemokine ntt | CX3CL1 | CXC3C | FKN | Fractalkine | Neurotactin | CXC3 | NTN | SCYD1 | Small-inducible cytokine D1
UniProt Number
P78423
SwissProt
P78423
Recommended Dilution
ApplicationDilution
WB1:1000 - 1:2000

Target

Target
Human CX3CL1 / Fractalkine
Antigen Species
Human
Epitope
This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with Fractalkine from other sources has not been determined.
Gene Name
CX3CL1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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