LSBio
Polyclonal Rabbit anti-Human CSPG4 / NG2 Antibody (IHC, IF, WB)

Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod

Polyclonal Rabbit anti-Human CSPG4 / NG2 Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human CSPG4 / NG2 antibodies →
$288.00each
Catalog Number: LS-C803040-100

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Overview

Description
NG2 antibody LS-C803040 is an unconjugated rabbit polyclonal antibody to NG2 (CSPG4) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CSPG4 | MCSP | MEL-CSPG | MSK16 | NG2 | HMW-MAA | MCSPG
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q6UVK1
SwissProt
Q6UVK1

Target

Target
Human CSPG4 / NG2
Antigen Species
Human
Epitope
NG2 Antibody detects endogenous levels of NG2.
Gene Name
CSPG4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthesized peptide derived from human NG2.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS pH 7.4;0.02% sodium azide;50% glycerol;150 mM NaCl
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS|150 mM NaCl

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