LSBio
Polyclonal Rabbit anti-Human CKM / Creatine Kinase MM Antibody (IHC, WB)

IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody. Creatine Kinase MM was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Creatine Kinase MM Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Polyclonal Rabbit anti-Human CKM / Creatine Kinase MM Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
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$480.00each
Catalog Number: LS-C756578-100

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Overview

Description
Creatine Kinase MM antibody LS-C756578 is an unconjugated rabbit polyclonal antibody to Creatine Kinase MM (CKM) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Application
ELISA, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CK-MB | CKMM | Creatine kinase M-type | Creatine kinase, muscle | CKM | M-CK | Creatine kinase M chain
Recommended Dilution: ELISA
0.1 - 0.5 ug/ml
Recommended Dilution: IHC-P
0.5 - 1 µg/ml
Recommended Dilution: WB
0.1 - 0.5 µg/ml
UniProt Number
P06732
SwissProt
P06732

Target

Target
Human CKM / Creatine Kinase MM
Antigen Species
Hu
Epitope
No cross reactivity with other proteins.
Gene Name
CKM

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
E. coli-derived human Creatine Kinase MM recombinant protein (Position: F3-K381).
Immunogen Type
Recom

Format

Conjugate/Label
Unconj
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer
NaCl

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