LSBio
Polyclonal Rabbit anti-Human CD5 Antibody (IHC, WB)

Flow Cytometry analysis of EL-4 cells using anti-CD5 antibody. Overlay histogram showing EL-4 cells stained with anti-CD5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Polyclonal Rabbit anti-Human CD5 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
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$480.00each
Catalog Number: LS-C756407-100

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Overview

Description
CD5 antibody LS-C756407 is an unconjugated rabbit polyclonal antibody to CD5 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, ICC, IHC and WB.
Application
ELISA, FC, ICC, IHC-F, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD5 | CD5 antigen | CD5 antigen (p56-62) | CD5 molecule | Lymphocyte antigen T1/Leu-1 | LEU1 | T1
Recommended Dilution: ELISA
0.1 - 0.5 ug/ml
Recommended Dilution: FC
1 - 3 µg/10E6 cells
Recommended Dilution: IF/ICC
0.5 - 1 µg/ml
Recommended Dilution: IHC-F
0.5 - 1 µg/ml
Recommended Dilution: IHC-P
0.5 - 1 µg/ml
Recommended Dilution: WB
0.1 - 0.5 µg/ml
UniProt Number
P06127
SwissProt
P06127

Target

Target
Human CD5
Antigen Species
Hu
Epitope
No cross reactivity with other proteins.
Gene Name
CD5

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
E. coli-derived human CD5 recombinant protein (Position: R25-L495).
Immunogen Type
Recom

Format

Conjugate/Label
Unconj
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer
NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.